It is located in the building of the former Anatomical Theater of the Faculty of Medicine of Kyiv University, which was built according to the project of the architect Alexander Beretti and on the initiative of the outstanding Kiev anatomist Professor Alexander Petrovich Walter in 1853. The building is a monument of architecture of the XIX century, built in the style of late classicism.

Anatomical Theater of the Kiev University of St. Volodymyra
The idea of creating a museum of medicine in Kiev arose in the early 1960s. The main role in its emergence and implementation belongs to the professor of the Department of Social Hygiene and Health Organization of the Kyiv Medical Institute named after him. O. O. Bogomolets, to the eminent Ukrainian historian of medicine Alexander Abramovich Grando (1919—2004). The initiative group for the search for future museum exhibits and materials for the museum has been working on a public basis for a long time. The main custodian of the funds was Leonid Mezhyrov.

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Until 2004, the director of the museum was its founder, Alexander Grando. From 2005 to the present, the museum is headed by Honored Doctor of Ukraine, Doctor of Medical Sciences Vadim Shipulin.

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The museum presents the development of medicine in Ukraine from ancient times to the present day. Its exposition consists of photos, books, instruments, anatomical preparations, personal belongings of prominent Ukrainian doctors, medicines, medical and pharmaceutical devices.

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Attention is paid to non-traditional architectural, artistic and technical, in particular audiovisual means of showing. Used scientific, methodological and documentary materials and exhibits in combination with architectural, artistic, technical and audio-visual means. Anatoly Utash and Volodymyr Kivlyuk were responsible for engineering and technical solutions, and Albert Kryzopolsky was responsible for the architectural and artistic project, the design of exhibition halls and showcases.

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The museum has created original interiors with portrait figures of famous scientists and doctors, and dioramas dedicated to important events in Ukrainian medicine. The author of portrait figures made of dental plastics is the artist and sculptor Spartak Brittan, Honored Worker of Culture of Ukraine.

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Works of Ukrainian fine art related to medical topics are also widely represented.

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The National Museum of Medicine of Ukraine is one of the largest medical museums in Europe. The work on the creation of this museum was in 1983 awarded the State Prize of Ukraine in the field of science and technology. The Decree of the President of Ukraine of February 15, 1999 granted the status of National.

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For about 25 years, the National Museum of Medicine of Ukraine has been one of the active members of the European Association of Museums of the History of Medical Sciences. The museum is known for its publishing, educational, exhibition activities.

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